I would suggest not to use the pen drive as it may overwrite the drive data and you will lose your data permanently. To get your lost data back you need to download the data recovery software ( Note: there are a number of data recovery software you may download as per your requirements such as Recuva, Stellar Phoenix, etc. ) connect your pen drive and run the data recovery tool to recover your lost stuff. Also would like to tell you that once the data deleted from the external drive, then there is no manual solution available for recovering the lost data such as recycle bin, etc.
