Heh, I never tried that specifically before now. But I just started a game, made new quicksave and then launched an airplane and took it up to 1000 meters above sea level and tryed to reload. It loaded the wrong save (not the one I made in space center just after I started the game) but it loaded alright.
Could be, because I was experimenting in all circumstances (launching several versions of same rocket or trying to get perfect atmospheric breaking) which involves lot of loading and reloading. Now the save file...I have not idea if it large or small, but persistance SFS file size is 3.8 MB. Judging by stuff happening in my game (55 objects including flags and asteroids are being tracked), I don't think it should be counted as "large".